Isolation and bioenergetic characterization of mitochondria fromPichia pastoris
نویسندگان
چکیده
منابع مشابه
Bioenergetic Evolution in Proteobacteria and Mitochondria
Mitochondria are the energy-producing organelles of our cells and derive from bacterial ancestors that became endosymbionts of microorganisms from a different lineage, together with which they formed eukaryotic cells. For a long time it has remained unclear from which bacteria mitochondria actually evolved, even if these organisms in all likelihood originated from the α lineage of proteobacteri...
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Mitochondria were isolated from autotrophically grown Chlamydomonas reinhardtii cell-wall-less mutant CW 92. The cells were broken by vortexing with glass beads, and the mitochondria were collected by differential centrifugation and purified on a Percoll gradient. The isolated mitochondria oxidized malate, pyruvate, succinate, NADH, and [alpha]-ketoglutarate. Respiratory control was obtained wi...
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ژورنال
عنوان ژورنال: Yeast
سال: 2006
ISSN: 0749-503X,1097-0061
DOI: 10.1002/yea.1355